Rapid Method to Determine Labeling Specificity of Radioactive Enteroviruses

Rapid method to determine labeling specificity of radioactive enteroviruses.

The specificity of labeling enteroviruses with (32)P-labeled NaH(2)PO(4) or (14)C-leucine can be determined by comparing the percent adsorption of infective particles and radioactivity to membrane (Millipore) filters.

A rapid method to determine the isotype and specificity of coproantibodies in mice infected with Trichinella or fed cholera toxin.

A method was developed to isolate and test coproantibodies from mice infected with Trichinella or mice fed cholera toxin. It was rapid as results were obtained within 3 h, and it was applicable to a single faecal pellet. It reduced the number of experimental animals required 7-fold, and reduced variability, since samples were obtained from the same experimental animal over time. As it was nonin...

A Method for Rapid Microsynthesis of Radioactive Cholesterol Esters.

WITH THE growing interest in the metabolism of cholesterol esters, procedures have been developed which frequently make desirable the use of chemically pure compounds. Some of the radioactive cholesterol esters are either commercially unavailable or expensive when made to order. The high cost of the starting materials (cholesterol-4-CI4, pure fatty acids) makes it desirable to have a good yield...

A rapid method of non radioactive Northern blot analysis.

The electrophoretic determination of RNA size by Northern blot analysis is an important procedure that has required to date the use of radiolabelled cDNA and cRNA probes. Here, we describe a new method which allows the size of an mRNA to be visualized by use of oligonucleotide probes directly labelled with alkaline phosphatase (AP). One advantage compared to radiolabelled probes is that AP-labe...

Non-radioactive Labeling of DNA and RNA